977 Résultats pour : « STEMCELL Technologies »

Supplier: STEMCELL Technologies

Serum-free medium for culture and expansion of hematopoietic cells.

Supplier: STEMCELL Technologies

Methylcellulose-based medium with recombinant cytokines for human cells. 1×100 ml bottle.

Supplier: STEMCELL Technologies

Semi-solid methylcellulose-based hybridoma selection and cloning medium without HAT (serum-containing).

Supplier: STEMCELL Technologies

Interleukin 13 (IL-13) is a cytokine important in type 2 immune responses and is expressed by T helper type 2 (Th2) cells and group 2 innate lymphoid cells (ILC2s) (Pulendran and Artis). IL-13 binds a receptor composed of IL-4Ra and IL-13Ra1 or IL-13Ra2 (Wynn 2003). IL-13 receptor is expressed on B cells and promotes B cell proliferation, induces class switching to IgG4 and IgE, and functions in the recruitment and activation of IgE-producing B cells (Hershey). The receptor is also expressed on basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells (Hershey). Signaling through the IL-13 receptor activates the JAK/STAT and IRS-1/IRS-2 pathways. in vivo, IL-13 has a role in resistance to extracellular helminth parasites by regulating gastrointestinal parasite expulsion, as well as in airway hyperresponsiveness, allergic inflammation, tissue remodeling, tumor cell growth, and fibrosis (Wynn 2015). Secreted IL-13 is a protein consisting of 112 amino acids with a molecular mass of 10 kDa (Hershey). Human IL-13 is not species-specific but has greater activity on human cells compared to mouse cells (Hershey).

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human, chimpanzee CD3, APC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220), unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal antibody against human, rhesus CD80 (B7-1), PE-conjugated.

Supplier: STEMCELL Technologies

For detection and measurement of human interferon-gamma.

Supplier: STEMCELL Technologies

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) Ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and in the development of the immune system (Hannum et al.). Flt3/Flk-2 Ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive CD34+ hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 Ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with the interleukins IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, and GM-CSF and TNF-α, Flt3/Flk-2 Ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 Ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler andamp; Quentmeier; Wodnar-Filipowicz). Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler andamp; Quentmeier; Hannum et al.).

Supplier: STEMCELL Technologies

For detection and measurement of mouse tumor necrosis factor-alpha.

Supplier: STEMCELL Technologies

A cellular protein for detection of apoptotic cells, APC-conjugated.

Supplier: STEMCELL Technologies

The EasySep™ Multistand allows the separation of up to four samples at one time.

Supplier: STEMCELL Technologies

Medium with cytokines for culture of human and mouse CFU-Mk. 24×2 ml vials.

Supplier: STEMCELL Technologies

The platelet-derived growth factor (PDGF) family has five heparin-binding members that assemble into four homodimers (PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD) and one heterodimer (PDGF-AB; Li and Eriksson). PDGF signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin such as fibroblasts and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-CC is secreted as a latent growth factor and requires activation by proteolytic processing (Li and Eriksson). PDGF-CC binds to PDGFRα homodimers and PDGFRαβ heterodimers, but not to PDGFRβ homodimers (Li and Eriksson). PDGF-CC is an angiogenic factor that stimulates coronary artery smooth muscle cell proliferation and plays a role in cardiovascular development (Gilbertson et al.). PDGF-CC is also expressed in many tumors and plays a role in tumorigenesis (Zwerner and May).

Supplier: STEMCELL Technologies

CD40 ligand is a type II transmembrane glycoprotein that belongs to the tumor necrosis factor (TNF) superfamily (Quezada et al.). CD40 ligand forms a bioactive homotrimer that exists as both soluble and membrane-bound forms (Khandekar et al.). CD40 ligand is expressed on T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. Its receptor, CD40, is expressed on B cells, dendritic cells, macrophages, monocytes, platelets, endothelial cells, and epithelial cells (van Kooten and Banchereau). Binding of CD40 ligand to CD40 stimulates B cell proliferation, immunoglobulin class switching, antibody secretion, and T cell-dependent humoral responses. Dysregulation of CD40 ligand contributes to immune deficiency in HIV and AIDS (Rickert et al.). CD40 ligand has also been linked to the pathology of atherosclerosis, atherothrombosis, and restenosis (Hassan et al.).

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of human cells labeled with PE-conjugated antibodies.

Supplier: STEMCELL Technologies

Supplement (50X) without vitamin A for the culture of neural progenitor cells.

Supplier: STEMCELL Technologies

Fibroblast growth factor 4 (FGF-4) is a member of the FGF superfamily (Beenken and Mohammadi). FGF-4 is expressed in pluripotent stem cells and is implicated in various stages of development and morphogenesis in a variety of organisms (Kosaka et al. 2009). FGF-4 has been shown to have an ability to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain (Kosaka et al. 2006). FGF-4 has also been shown to increase the proliferation rate of mesenchymal stem cells (Farré et al.). FGF-4 supports the maintenance and self-renewal properties of human embryonic stem cells and also promotes the proliferation of these cells (Mayshar et al.). In the mouse, the Fgf4 gene also supports proliferation of the inner cell mass (ICM) and postimplantation embryos (Feldman et al.).

Supplier: STEMCELL Technologies

Serum-free supplements for differentiation of human pluripotent stem cells (hPSCs) to myogenic progenitor cells.

Supplier: STEMCELL Technologies

Apolipoprotein H (apo H) has been shown to promote the coagulation of blood platelets by inhibiting thrombomodulin complex and inactivating protein C (Keeling et al.), but can also act as an anticoagulant by binding thrombin and inhibiting its procoagulant effects (Pozzi et al.). Belonging to the lipid-binding apolipoprotein family, within the lipocalin superfamily, apo H is a protein constituent of plasma, with a high affinity for negatively charged phospholipids. The structure of apo H reveals four N-terminal complement control protein (CCP) modules, also known as 'sushi' domains, and a distinct fifth C-terminal domain with four antiparallel beta sheets, two alpha-helices, and an extended loop (Schwarzenbacher et al.). Apo H is the main antigen implicated in antiphospholipid syndrome (APS), an autoimmune condition involving pregnancy complications and vascular thrombosis (Brusch). Studies have also reported that Apo H is involved in the progression of atherosclerosis (Harats and George). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, apolipoprotein H from STEMCELL comes lyophilized with ≥93% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1.0 EU/μg protein.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV1 (gD) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV1 (gD) Peptide Pool is a lyophilized mixture of 96 peptides from envelope glycoprotein D of human herpesvirus 1 (HHV1; strain Patton), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 394 on gD. gD is required at the first stage of virus-induced membrane fusion (Subramanian and Geraghty), which likely occurs via binding to receptors such as nectin-1 (Giovine et al.) and HVEM (Connolly et al.) on cell surfaces. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Kit for the detection of extracellular vesicles using CD9, CD63, and CD81 markers.

Supplier: STEMCELL Technologies

Stimulating T cells with HHV6 (U54) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HHV6 (U54) peptide pool is a lyophilized mixture of 112 peptides from protein U54 of human herpesvirus 6B (HHV-6B; strain Z29), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 459 on U54. U54 is a tegument transactivator and is suggested to be a homolog of the CMV UL82/83 (pp65/72K) protein (Isegawa et al.; Nicholas). In infected T cells, U54 has been shown to inhibit gene transcription of interleukin 2 (IL-2), the T cell growth factor, which likely contributes to viral persistence (Iampietro et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with SARS-CoV-2 (Spike Protein) Omicron/B.1.1.529 Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This pool is provided as two lyophilized mixtures (subpools) from the spike glycoprotein of SARS-CoV-2 Omicron variant (B.1.1.529), which contain 158 and 157 peptides, respectively, for a total of 315 peptides. They consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1270 on the spike protein. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann et al.; Walls et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

DNase- and RNase-free water for molecular biology applications.

Supplier: STEMCELL Technologies

Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Martin et al.; Kent et al.). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). SCF is also a primary growth and activation factor for mast cells and eosinophils. SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain.

Supplier: STEMCELL Technologies

Activin A is a member of the transforming growth factor beta (TGF-β) family of proteins produced by many cell types throughout development (Gurdon et al.). It is a disulfide-linked homodimer (two beta-A chains) that binds to heteromeric complexes of a type I (Act RI-A and Act RI-B) and a type II (Act RII-A and Act RII-B) serine-threonine kinase receptor (Attisano et al.). Activins primarily signal through SMAD2/3 proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (McDowell et al.). Activin A maintains the undifferentiated state of human embryonic stem cells (James et al.; Xiao et al.) and also facilitates differentiation of human embryonic stem cells into definitive endoderm (D’Amour et al.).

Supplier: STEMCELL Technologies

Culture medium kit for initiation, growth, and differentiation of human liver organoids.

Supplier: STEMCELL Technologies

Methylcellulose-based medium with recombinant cytokines (including EPO) for mouse cells. 1×100 ml bottle.

Supplier: STEMCELL Technologies

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-κB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immunosuppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová andamp; Hosek). Other effects of TNF-α include vasodilatation and edema formation.