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Primary Human Osteoblasts (HOB), PromoCell

Supplier: Promocell

Primary Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium.

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Primary Human Skeletal Muscle Cells (SkMC), PromoCell

Supplier: Promocell

Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin. New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

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Accessories for Endothelial Cell Growth Medium, PromoCell

Supplier: Promocell

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Human Dermal Blood Endothelial Cells (HDBEC), PromoCell

Supplier: Promocell

Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.) The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are > 95% CD31 positive and stain positive for von Willebrand factor. In addition, they are > 90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.

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Human Aortic Smooth Muscle Cells (HAoSMC), PromoCell

Supplier: Promocell

Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process.

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Melanocyte Growth Medium, PromoCell

Supplier: Promocell

The PromoCell Melanocyte Growth Medium is a serum-free, PMA (Phorbol Myristate Acetate) containing medium optimized for the cultivation of human melanocytes

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Keratinocyte Growth Medium 2, PromoCell

Supplier: Promocell

PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed for the cultivation of epidermal keratinocytes without feeder cells

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Normal Human Epidermal Melanocytes (NHEM), PromoCell

Supplier: Promocell

Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs. They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation.