203 Résultats pour : « Promocell »

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Calcium chloride solution allows the adjustment of the optimal amount of calcium required by the cells. Calcium chloride (CaCl₂) solution is prepared as a solution from highly pure tissue culture tested calcium chloride and cell culture-grade water.

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Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells. Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.

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Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request). Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes.

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Primary Human Pulmonary Fibroblasts (HPF) are isolated from human lung tissue

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Primary Human Umbilical Artery Smooth Muscle Cells (HUASMC) are isolated from the arteries of the umbilical cord and stain positive for smooth muscle α-actin

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Primary Human Tracheal Epithelial Cells (HTEpC) are isolated from the surface epithelium of human trachea and stain positive for cytokeratin.

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PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

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This DetachKit is used for gentle and effective detachment of adherent primary human cells in routine subculturing.

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The Macrophage Detachment Solution DXF is a non-enzymatic reagent especially optimized for the gentle detachment of strongly adherent macrophages.

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PromoCell Mesenchymal Stem Cell Media were developed for the in vitro expansion and directed differentiation of mesenchymal stem cells (MSC) from bone marrow, the umbilical cord matrix (Wharton´s Jelly) and adipose tissue

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Endothelial Cell Growth Medium and Endothelial Cell Growth Medium 2 are low-serum (2% V/V) media optimized for the cultivation of endothelial cells from large blood vessels

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PromoCell Endothelial Cell Growth Medium MV and Endothelial Cell Growth Medium MV2 were developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta

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PromoCell hMSC-BM are harvested from normal human bone marrow. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, osteoblasts, and neuronal cells.

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Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area. They stain positive for cytokeratin.

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The PromoCell Myocyte Growth Medium is a low serum (5% V/V) medium supplemented with well chosen hormones and growth factors

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Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of juvenile foreskin and adult skin (different locations) from a single donor.
(Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.) The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: > 95% of the cells are CD31 positive  and podoplanin positive. In addition, the cells stain positive for Prox-1.

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Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated from human dermis originating from the lateral scalp. Information on donor hair and skin color is available for each lot. HFDPC stain positive for alkaline phosphatase.

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These solutions are prepared from raw materials of the highest quality using tissue culture grade water.

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The PromoCell Preadipocyte Growth Medium is a low-serum (5% V/V) medium optimized for the expansion of human preadipocytes

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Small Airway Epith Cell Growth Medium SupplMix; 1 mix

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The PromoCell Fibroblast Growth Medium is a serum-free medium optimized for the cultivation of neonatal and juvenile fibroblasts

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PromoCell Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.

Supplier: Promocell

Primary Human Bronchial Epithelial Cells (HBEpC) are isolated from the surface epithelium of human bronchi and stain positive for cytokeratin

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Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.

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Primary Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request).

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Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.

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Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue