"Cube Biotech"

Supplier: Cube Biotech

Elution of Rho1D4-tagged protein from matching purification beads.

Supplier: Cube Biotech

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-55 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-55 is named from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-55 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiencies, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: Cube Biotech

SMALP BZs are synthesized via RAFT polymerization, achieving a better-defined polymer size and a low dispersity.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

PolyHunter agarose was developed for the depletion of synthetic polymers used in solubilization steps.

Supplier: Cube Biotech

HiCap StrepTactin® Compact Cartridge is a FPLC-ready column that is pre-packed with our PureCube HiCap StrepTactin® agarose resin.

Supplier: Cube Biotech

PureCube Zn-NTA magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.

Supplier: Cube Biotech

HighSpec Rho1D4 MagBeads are a suspension of 30 µm magnetic agarose beads coupled to an anti-Rho1D4 antibody.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

HighSpec Rho1D4 Agarose resin is a suspension of 40 µm agarose beads, coupled to an anti-Rho1D4 antibody. It is meant for protein purification assays of the highest possible purity, exploiting the specificity of an antibody to its antigen.

Supplier: Cube Biotech

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: Cube Biotech

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: Cube Biotech

Our Rho1D4 starter set 3 combines our HighSpec Rho1D4 compact cartridge and the Rho1D4 elution peptide.

Supplier: Cube Biotech

The Rho1D4 starters set 2 combines our HighSpec Rho1D4 MagBeads and the Rho1D4 elution peptide.

Supplier: Cube Biotech

CUBE MAGBEAD SEPARATOR 4 MICROTUBES PK2