251 Results for: "CUBE BIOTECH"

Supplier: CUBE BIOTECH

Membrane scaffold protein, mouse origin.

Supplier: CUBE BIOTECH

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

Our PureCube Ni-IDA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.

Supplier: CUBE BIOTECH

Membrane scaffold protein, human origin.

Supplier: CUBE BIOTECH

PureCube maleimide MagBeads XL are your best option to couple biomolecules carrying thiol (SH) groups.

Supplier: CUBE BIOTECH

Our PureCube Co-NTA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used for the purification of active His-tagged proteins from cells.

Supplier: CUBE BIOTECH

PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Supplier: CUBE BIOTECH

Our PureCube Ni-IDA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used to purify active His-tagged proteins from cells and extremely viscous cell media.

Supplier: CUBE BIOTECH

Empty 1-step batch columns are ideally suited for batch purifications. They can be filled with your preferred affinity resin, allowing binding, washing, and elution in one spin column.

Supplier: CUBE BIOTECH

The HighSpec Rho1D4 Compact cartridge is a pre-packed FPLC column containing our HighSpec Rho1D4 Agarose resin.

Supplier: CUBE BIOTECH

This is an antibody specifically for targeting a Rho1D4-(TETSQVAPA)-tag on a protein.

Supplier: CUBE BIOTECH

DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.

Supplier: CUBE BIOTECH

Empty PureCube Compact cartridges can be filled with your affinity resin of choice.

Supplier: CUBE BIOTECH

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Supplier: CUBE BIOTECH

The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

Supplier: CUBE BIOTECH

PureCube NHS (N-hydroxy succinimide) Agarose resin is your best option to couple biomolecules with free-standing amine groups.

Supplier: CUBE BIOTECH

PureCube amine carboxy agarose MagBeads are your best option to couple biomolecules that carry free-standing Amine (NH₂) groups.

Supplier: CUBE BIOTECH

Phosphocholines, or Fos-cholines, are a novel class of detergents that is well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer Fos-cholines with chain lengths from C-9 to C-16.

Supplier: CUBE BIOTECH

Octylthioglucoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer glucosides with chain lengths from C-8 to C-10.

Supplier: CUBE BIOTECH

Decylmaltoside are well suited for the solubilization, stabilization, and purification of membrane proteins. For screening of the best-suited detergent for a given application, we offer maltosides with chain lengths from C-9 to C-13.

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 6-55 - are highly-alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 6-55 gets its name from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general, lighter AASTYs, like 6-55 tend to be more aggressive, while heavier AASTYs, such as 11-45 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiency, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process, we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

AASTYs (Acrylic acid-co-styrenes) - like AASTY 11-55 - are highly alternating copolymers, well-suited for generating native lipid nanodiscs. They are a 2022 novel developed series for membrane protein solubilization & stabilization. AASTY 11-55 is named from its molecular weight and Acrylic Acid : Styrene Ratio. These varying ratios of acrylic acid to styrene contribute to the hydrophilic properties of our AASTYs. In general lighter AASTYs, like 6-45 tend to be more aggressive, while heavier AASTYs, such as 11-55 show higher thermodynamic stability.

The exact composition of AASTY copolymers shows different extraction efficiencies, depending on the lipid composition of the lipid bilayers being formulated into nanodiscs. As AASTY is made by controlled radical polymerization techniques, the dispersity of polymer molecular weight distribution is low, and the molecular weights are controlled. This means that excess AASTY copolymer can be removed by dialysis after nanodisc formation. Based on previous findings on SMA, it is the expectation that AASTY of different molecular weights will display different rates of nanodisc formation, extraction efficacy, and stability of resulting nanodiscs.

Every membrane protein solubilization needs to undergo a screening process before. The characteristic phospholipid environment surrounding the different membrane proteins in question performs differently well with each polymer. To support you in this process we offer a handy Screening Kit for AASTYs to test them all. Additionally, we recommend the two following publications if you would like to get further information: Smith et al. 2020 & Timcenko et al. 2022

Supplier: CUBE BIOTECH

With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations. A good publication to read even more details about DIBMA is Oluwole et al. 2017.