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GelRed™ and GelGreen™ are a new generation of fluorescent nucleic acid gel stains designed to replace the highly toxic ethidium bromide (EtBr)

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is prepared by labeling IgG with CF350. CF350 is a blue fluorescent dye spectrally similar to AMCA. However, compared to AMCA, CF350 is more water-soluble, more photostable and at least 50% more fluorescent on proteins.

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is labeled with the deep-red fluorescent dye CF594 which is spectrally similar to Alexa Fluor 594 and Texas Red dyes but yields much brighter protein conjugates. CF594 has excellent photostability ideal for demanding applications, such as multi-color imaging.

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is labeled w/far-red fluorescent dye CF633 which is brighter than Alexa Fluor 647 & other spectrally similar dyes. More importantly, CF633 has photostability making it the best choice for demanding applications, such as confocal & single molecular imaging.

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DiOC6(3) (3,3′-Dihexyloxacarbocyanine,iodide) is a green fluorescent membrane dye that has been used to stain the ER in both live and fixed cells. The dye has also been used to study structural interactions and dynamics of the ER in neurons and yeast. In addition, the dye is widely used as a membrane potential dye.

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JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.

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RH421 is a fast responding membrane potential dye, exhibiting >20% fluorescence change per 100 mV on neuroblastoma cells.

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TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.

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NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.

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Reactive dyes containing an amine functional group.

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SR101-PEO8 SE (Sulforhodamine 101-PEO8-propionic acid succinimydyl ester; Texas Red®-PEO8 SE) has an approximately 32 Angstrom water-soluble PEG spacer that separates the dye from the amine-reactive SE group.

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Biotin-20-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. Biotin-dUTP conjugates are available wtih 11-carbon, 16-carbon, or 20-carbon linker lengths.

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Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains. Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

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BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.

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is a popular nucleic acid gel stain. the dye binds to dna with 10-fold fluorescence enhancement and has little sequence preference. eb is a potent mutagen, biotium offer eb in h2o as a safer alternative to the more hazardous powdery form.

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Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies. Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens. We offer a highly purified form of acridine orange while most of the other commercially available grades of AO are either in zinc chloride complex form or of low purity.

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reacts with nitric oxide (NO) to form a red (em > 580 nm) fluorescent precipitate. DAA probe has been used to detect NO concentration in rat retinas after injury to the optic nerve.

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with the green fluorescent dye CF488A. CF488A is a superior alternative to FITC (or fluorescein) due to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488, and more specific.

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with the green fluorescent dye CF488A. CF488A is a superior alternative to FITC (or fluorescein) due to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488, and more specific.

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with an orange-red fluorescent dye CF555, one of an outstanding series of CF dyes developed by Biotium. CF dyes are superior for antibody labeling by having combined advantages in brightness, photostability, and specificity.

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Kits to detect live and dead bacteria, and perform Gram stain using fluorescent wheat germ agglutinin (WGA) conjugate.

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Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

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is prepared by labeling affinity purified IgG (H+L) w/far-red fluorescent dye CF647, one of a series of CF dyes developed by Biotium. CF dyes are superior for antibody labeling by having combined advantages in brightness, photostability & specificity.

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is prepared by labeling affinity purified IgG (H+L) with an orange-red fluorescent dye CF555, one of an outst&ing series of CF dyes developed by Biotium. CF dyes are superior for antibody labeling by having combined advantages in brightness, photostability, & specificity.

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was conjugated to biotin. Useful for three step labelings with conjugated streptavidins or avidins.

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NucView® 488 Caspase-3 substrate kits for real-time apoptosis monitoring in live cells are bundled with the NucView® caspase-3 substrate and various apoptosis or necrosis markers for convenient multi-color experiments.

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A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

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Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

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This conjugate is prepared by labeling F(ab’)2 fragment of goat anti-mouse IgG (H+L) with CF™647 dye. CF™ dyes offer advantages in brightness, photostability, and specificity compared to other fluorescent dyes. Far-red fluorescent CF™647 (Ex/Em 650/665 nm) is brighter than Cy®5 and an excellent alternative to Alexa Fluor® 647 for antibody conjugates.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Cy®5 is a registered trademark of GE Healthcare.

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is prepared by labeling IgG with CF568, a red fluorescent dye with an excitation spectrum optimal for the 568 nm laser line. Antibody conjugates of the dye are brighter & more photostable than those of Alexa Fluor 568, making CF568 a better choice for demanding applications.