phi29-XT WGA Kit

The phi29-XT WGA Kit has been optimized for sensitive, fast, and robust whole genome amplification (WGA), an isothermal method for amplification of an entire genome, starting from femtogram quantities of DNA and resulting in microgram quantities of amplified products.

• High sensitivity: Amplify from as little as 10 fg of input DNA
• Powered by phi29-XT DNA Polymerase, an engineered polymerase
• Flexible protocol allows for different types of input material
• Simple workflow produces high yield in a short reaction time (less than 2 hours)
• Whole genome representation and uniform GC coverage for human and microbial DNA

The kit features phi29-XT DNA polymerase, an engineered polymerase with improved thermostability, sensitivity, and generates higher yield in a shorter reaction time than wild-type phi29 DNA Polymerase. Also included are exonuclease-resistant random primers (containing phosphorothioate bonds) to universally amplify any DNA sequence, neutralization buffer, and dNTPs.

Input material can be purified genomic DNA, cells, or microbiomes. WGA enables several downstream applications, including single cell whole genome sequencing (WGS), genomic DNA enrichment, and environmental DNA (eDNA) enrichment and sequencing.