Extracta DBS, Quantabio

Extracta DBS is a rapid and easy to use extractions reagent optimized for the extraction of DNA from dried blood spot punches

• Optimized for DNA extraction from dried blood spot punches
• Single reagent for PCR-ready DNA in less than 30 minutes
• Maximized assay sensitivity, lower Ct’s, when combined with Quantabio ToughMix
• Compatible with high-throughput automation for PCR, qPCR and NGS applications
• Reliable, Consistent Lot-to-Lot Performance

Extracta DBS is a ready-to-use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman 903 filter paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing (1-3) reagents. Application of Extracta DBS with PerfeCta® qPCR ToughMix® or PerfeCta® MultiPlex qPCR ToughMix enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

The resulting extract is immediately usable for downstream applications such as real-time PCR.