Sterikon® Plus Bioindicators, MilliporeSigma

A rapid and easy-to-use method for verifying steam sterilization.

Indicators consist of an ampule containing nutrient broth, sugar, a pH indicator, and 2mL of spores from an apathogenic organism: Bacillus stearothermophilus ATCC 7953

Use to monitor the effectiveness of steam sterilization at 121°C (250°F) for 15 minutes, as well as to confirm that the prescribed temperature is reached throughout the chamber and remains constant for the entire 15 minutes. Autoclaves with capacities of up to 250 L require at least two ampules, while larger ones require at least six.

Note: Not intended for monitoring sterilization of medical devices — for laboratory use only. Avoid use of the ampules at temperatures exceeding 125°C (257°F) to prevent damage to the bioindicator, resulting in color changes without increased turbidity.

Simply place ampules in the autoclave along with the batch to be sterilized, and incubate afterwards. A color change of the ampule contents clearly indicates whether sterilization was successful.

Certifications: Sterikon Plus meets USP XXIV guidelines for monitoring the effectiveness of steam sterilization.