Thymus Lysate, ProSci
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Product Details & Documents
Thymus tissue lysate (Human) was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors)
Thymus tissue lysate (Mouse and Rat) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Spécifications
Spécifications
- Format de cellule:Cryopreserved
- Désignation:Normal
- Taille:0.1 mg
- Source:Rat
- Tissue:Thymus
- Cat. No.:10062-268
- Storage conditions:Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Concentration:2 mg/mL
- Tested applications:WB
- Storage buffer:Thymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
Spécifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark