Pierce™ RIPA Buffer, Thermo Scientific
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Product Details & Documents
Thermo Scientific RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
- Compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
- Enables extraction of cytoplasmic, membrane and nuclear proteins making it very versatile
- Contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells.
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
Specifications
Specifications
- Désignation:RIPA Lysis and Extraction Buffer, 250 mL
Specifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark