Pierce™ Blocker™ BSA Blocking Buffer, Thermo Scientific

Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

• Easy to use
• Convenient and saves storage space
• Purified protein provides fewer chances of cross reaction with assay components

This 10% (w/v) solution of high quality BSA that is useful for saturating excess protein binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) in 1X Tris-buffered saline for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than non fat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.