PerfeCTa® MultiPlex qPCR SuperMix

This SuperMix is a 2x concentrated, ready-to-use reaction cocktail for real-time quantitative PCR (qPCR) that contains all components, except primers, probes, and templates

• Ultrapure, hot start AccuStart enzyme technology and anti-foaming technology
• Available in two versions: Universal and Low-ROX
• Maximum assay sensitivity and target precision with stringent, ultrapure, and high-yielding AccuStart II hot start Taq DNA polymerase
• Broad linear detection range with cDNA or genomic DNA templates
• Enables highly sensitive 4 target detection assays with coamplified, robust internal positive controls
• Supports efficient vortex mixing with proprietary anti-foaming technology

A key component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.