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- Assay duration:Multiple steps
- Assay Type:Indirect
- Conjugate ELISA:Unconjugated
- Format:Pre-coated
- Host:Goat
- Primary antibody reactivity:Human
- Target protein:Nipah virus fusion glycoprotein
- Size:96 tests
This kit is developed for serologic test for human IgG titer of Anti-NiV F and G antibody in serum/plasma or purified human antibody (monoclonal and polyclonal) in vitro.
This product was tested to bind to monoclonal antibody RV41 (M72A69)
Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses (HNVs), which are members of the paramyxovirus family, including a number of pathogens causing disease in humans such as measles, mumps and parainfluenza viruses. After a primary infection, HeV and NiV enter the target cells with a receptor-binding glycoprotein (G) and a fusion glycoprotein (F). F and G are the main targets of vaccine development, associated with neutralizing antibody response and correlated of protection. A rapid and effective assay kit detecting the levels of anti-NiV F and G in human serum can facilitate research on antibodies produced in response to NiV infection or vaccine candidates.
Assay Principles: This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-Nipah virus (NiV) fusion glycoprotein (F and G) human IgG in serum. The kit consists of high-bind detachable 96-well plate pre-coated with F and G protein, positive control, negative control, an HRP-Anti-Human IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (4 simple steps):
1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37 °C for 1 hour or room temperature for 1.5 hour. Wash wells four times by wash solution (0.3 ml/well).
2. Dilute the Secondary antibody HRP-Anti-Human IgG by wash solution and add to each well (0.1 ml/well). Incubate at room temperature for 0.5 to 1 hour. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml/well) and incubate at room temperature for 10 to 30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
For research use only.