NxGen™ M-MulV Reverse Transcriptase, Biosearch Technologies

Lucigen’s NxGen™ M-MulV Reverse Transcriptase is outstanding in cDNA synthesis.

M-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3′→5′ proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.

Performance Specifications: 1) Unit Concentration: 200,000 U/mL. 2) Purity (SDS-PAGE): >99%. 3) SS Exonuclease: 2,000 U <5.0% released. 4) DS Exonuclease: 2,000 U <0.5% released. 5) Endonuclease: 2,000 U <10% converted. 5) E. coli 16S rDNA Contamination: 2,000 U <10 copies. 6) Storage: -20°C.

Source: A recombinant E. coli strain carrying the Moloney-Murine Leukemia Virus Reverse Transcriptase gene.

: Supplied at 200,000 U/mL in 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% NP-40 Alternative, 50% glycerol, pH 7.6 @ 25°C. Also provided is 10X M-MuLV RT Buffer which in 1X form is composed of 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol, pH 8.3 @ 25°C. 250,000 U is supplied as 5 × 50,000 U. Unit definition: 1 unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into acid insoluble material in 10 minutes at 37°C using poly r(A)/oligo (dT) as a substrate.