The Total RNA Mini and Maxi Tissue Kits are specially designed for purification of total RNA from a variety of animal tissues or cells

The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube. The method uses a detergent and chaotropic salt to lyse cells and inactivate RNase. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed following the wash step, the purified RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed within 30 minutes for the Mini Kit, and 60 minutes for the Maxi Kit. The purified RNA is ready for RT-PCR, Northern blotting, primer extension, and cDNA library construction.