E. cloni® Electrocompetent Cells, Biosearch Technologies
About this item
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields, and reliability, while decreasing costs. These cells are unrivalled in quality, efficiency, and exceptionally low costs. Lucigen's E. cloni cells provide solutions for a wide range of applications at remarkably economical prices.
Lucigen offers the following choices of strain: 1) E
And the following choices of efficiency: 1) E. cloni 10G SUPREME Electrocompetent Cells ≥4 x 1010 cfu/µg pUC. SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible. 2) E. cloni 10G & 10GF´ ELITE Electrocompetent Cells ≥2 x 1010 cfu/µg pUC. ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price. 3) E. cloni 10G CLASSIC Electrocompetent Cells ≥5 x 109 cfu/µg pUC. CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.
The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening. 2) E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
: Competent cells include Control DNA and Recovery Medium. Recovery medium can be ordered separately.
: Packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), or SixPacks (6 transformations per tube).
13 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields, and reliability, while decreasing costs. These cells are unrivalled in quality, efficiency, and exceptionally low costs. Lucigen's E. cloni cells provide solutions for a wide range of applications at remarkably economical prices.
Lucigen offers the following choices of strain: 1) E
And the following choices of efficiency: 1) E. cloni 10G SUPREME Electrocompetent Cells ≥4 x 1010 cfu/µg pUC. SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible. 2) E. cloni 10G & 10GF´ ELITE Electrocompetent Cells ≥2 x 1010 cfu/µg pUC. ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price. 3) E. cloni 10G CLASSIC Electrocompetent Cells ≥5 x 109 cfu/µg pUC. CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.
The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening. 2) E. cloni 10GF′ Competent Cells. Contain the F' plasmid for infection with M13 to produce ssDNA.
: Competent cells include Control DNA and Recovery Medium. Recovery medium can be ordered separately.
: Packaged as SOLOs (1 transformation per tube), DUOs (2 transformations per tube), or SixPacks (6 transformations per tube).
Specifications for Product Family
Specifications
- Catalog No:
- 89002-658
- 89002-666
- 89002-670
- 95024-012
- CA89005-074
- 89002-660
- 89002-662
- 89002-668
- 89002-672
- 89002-684
- CA89005-076
- 89002-664
- 89002-686
- 89047-690
- 95024-014
- Genotypes:
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 Δlac×74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 Δlac×74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Specifications
Product Family Options
Product Information
- DésignationTailleNo. of ReactionsPackagingAvailabilityPrice
- E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)12DuoLog in for availabilityEach$717.24Supplier Number: 60052-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.89002-658GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10GF' ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)12DuoLog in for availabilityEach$699.14Supplier Number: 60061-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-666GenotypesE. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 Δlac×74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA - E. cloni 10G SUPREME Electrocompetent Cells (≥4 x 10^10 cfu/µg DNA)12DuoLog in for availabilityEach$1,083.19Supplier Number: 60080-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-670GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G SUPREME Electrocompetent Cells (≥4 x 10^10 cfu/µg DNA)12SOLOLog in for availabilityEach$1,229.40Supplier Number: 60081-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.95024-012GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)12SOLOLog in for availabilityEach$2,270.74Supplier Number: 60051-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.CA89005-074Genotypes - E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)24DuoLog in for availabilityEach$1,204.39Supplier Number: 60052-2Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.89002-660GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)24Six packLog in for availabilityEach$937.95Supplier Number: 60052-3Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-662GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10GF' ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)24DuoLog in for availabilityEach$1,194.95Supplier Number: 60061-2Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.89002-668GenotypesE. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 Δlac×74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA - E. cloni 10G SUPREME Electrocompetent Cells (≥4 x 10^10 cfu/µg DNA)24DuoLog in for availabilityEach$1,979.30Supplier Number: 60080-2Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-672GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G CLASSIC Electrocompetent Cells (≥5 x 10^9 cfu/µg DNA)24Six packLog in for availabilityEach$807.99Supplier Number: 60117-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-684GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)24SOLODiscontinued-Supplier Number: -Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.CA89005-076Genotypes - E. cloni 10G ELITE Electrocompetent Cells (≥2 x 10^10 cfu/µg DNA)48Six packLog in for availabilityEach$1,534.04Supplier Number: 60052-4Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-664GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - E. cloni 10G CLASSIC Electrocompetent Cells (≥5 x 10^9 cfu/µg DNA)48Six packLog in for availabilityEach$1,422.75Supplier Number: 60117-2Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89002-686GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA - Discontinued-Supplier Number: -Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.95024-014GenotypesE. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Recovery Medium
Product Information
- DésignationTailleNo. of ReactionsPackagingAvailabilityPrice
- Supplier Number: 80026-1Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.89047-690Genotypes
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#: CA21905-026
Supplier: Kimberly-Clark