Corning® HEPES buffer solution (1 mol/L)

CA45000-690

Corning® HEPES buffer solution (1 mol/L)

CA45000-690

: 25-060-CI

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Pack type:
Glass bottle
Description:
HEPES buffer solution (1 mol/l)
Storage temperature:
Ambient
Cat. no.:
CA45000-690
Volume:
100 ml
Supplier No.:
25-060-CI

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.