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Specifications
- Description:Western blot analysis kit
- No. of Lab Groups:6
- Cat. no.:470120-900
- Supplier No.:317
Specifications
About this item
Protein identification is based on both antibody reactions and antigens in this Western Blot activity.
- Materials for Six Blots Included
- Instructions with Background Information
- Students Research Protein Identification
A denaturing SDS polyacrylamide gel separates the proteins, which are transferred or blotted to a nylon membrane. The membrane is then exposed sequentially to solutions containing the primary antibody, followed by a secondary antibody that is coupled to an enzyme.
The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band, and the molecular weights of the visible bands are measured using pre-stained protein markers.