G-Biosciences' HOOK™ BiotinQuant is designed for the estimation of biotin conjugation.

G-Biosciences' HOOK™ BiotinQuant™ uses HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye to rapidly estimate the mole-to-mole ratio of biotin to antibody or protein. The method of biotin incorporation estimation is based on the binding of avidin with HABA dye, which produces a color that can be read at 500 nm. The HABA-avidin complex can be displaced with free biotin or biotin conjugated with other molecules (proteins). Measuring the change in optical density of HABA-avidin complex with biotinylated proteins allows for accurate estimation of the molar ration of biotin conjugated to the protein/ antibody.

G-Biosciences' HOOK™ BiotinQuant™ is supplied with premixed, OneQuant™ HABA/Avidin aliquots to eliminate difficulties in weighing small amounts of reagents and ensuring reproducible estimations.