G-Biosciences' Immobilized Biotin Resin is designed for the high affinity chromatography purifications of avidin, streptavidin and Neutravidin protein.

G-Biosciences' Immobilized Biotin Resin consists of biotin coupled to 6% cross-linked agarose. Biotin, a 244 Dalton vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵M¯¹) and streptavidin. Biotin and avidin interaction is rapid, and once the bond is established, it can survive up to 3 M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8 M guanidine-hydrochloride at pH 1.5 or by boiling in SDS PAGE sample loading buffer.

G-Biosciences' Immobilized Biotin Resin is covalently coupled to limit leaching, and it is reusable at least 10 times.