G-Biosciences' Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm

Molecules can be coupled to the free amine by numerous amine-reactive methods. However, the use of the carbodiimide EDC allows coupling of free carboxyl groups. The resulting amide bond is highly stable and greatly reduces the chance of leaching of the affinity tag. The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification.

G-Biosciences' Carboxyl Coupling Resin has a very high capacity of immobilized DADPA and offers >60 µmole amine load for every ml of resin. This high capacity offers unsurpassed efficiency and capacity in the immobilization of carboxyl containing protiens. The Carboxyl Coupling Resin couples 1-2 mg of peptide per milliliter of resin. G-Biosciences' Carboxyl Immobilization kit can also be used for the immobilization of nucleic acids/ oligonucleotides through 5' phosphate groups.

Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.