It is an improved Coomassie Dye based protein assay based on the Bradford Protein Assay
CB™ Protein Assay is supplied with a simple to follow protocol and a ready to use reagent that does not require prefiltering or dilution. Simply mix the protein solution with CB™ Protein Dye and read optical density.
The protein-dye complexes reach a stable end point in 5 minutes. The CB™ Protein Assay is compatible with reducing agents and a wide variety of common laboratory agents. Note: The Coomassie dye based assay is not suitable if the protein solution contains higher than recommended concentration of detergents or other agents. CB™ Protein Assay is sensitive over the linear range of 0.5-50 µg. Suitable for 500 assays.
This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5 µg protein.
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