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- Description:InGel™ Silver
- Size:100 preps
- Cat. no.:CA82021-586
- Supplier No.:786-241
G-Biosciences' InGel™ Silver provides a complete set of reagents for the in gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS)
The protein spots are first excised from the silver stained gel and transferred to a proteomic grade tube. Silver stained gel pieces are washed with SilverOUT™ to remove inhibitory silver ions. The protein is then alkylated and reduced within the gel piece using the supplied aliquots of OneQuant™ DTT and OneQuant™ Iodoacetamide. The proteins are then digested within the gel using our Mass Spectrometry Grade Trypsin and proprietary Digestion Buffer.
The digested peptides are extracted with Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without any subsequent treatments or cleaning procedures.
G-Biosciences' InGel™ Silver kit is supplied with SilverOUT™ for the removal of silver ions, OneQuant™ DTT used as a reducing agent and supplied in single use aliquots to prevent contamination, OneQuant™ Iodoacetamide used as an alkylating agent, again in single use aliquots to prevent contamination, Mass Spectrometry Grade Trypsin, Trypsin Digestion Buffer, and Pep-Extract™ for high level peptide extraction.
The kit is specifically designed for use with silver stained protein spots/bands.