G-Biosciences' Well-Coated™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules

G-Biosciences' Well-Coated™ Amine Binding plates are maleic anhydride activated plates that react with primary amines to form amide bonds that are stable at pH≥7. Acidic conditions will hydrolyze the bonds releasing the peptide/ligand, therefore binding of peptide/ligand to plates should be performed at pH8-9 and the binding assays or ELISA should be performed at pH≥7.

The wells are coated to a 200µL depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.

The Well-Coated™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for binding assays.