Perfect-FOCUS™ is designed to clean and concentrate protein samples that give poor protein spot resolution during 2D electrophoresis

Protein solutions are treated with Perfect-FOCUS™ that quantitatively precipitates the proteins, which are subsequently collected by centrifugation. The non-protein interfering agents are washed away and the protein pellet is ready to be solubilized in an appropriate sample loading buffer.

The collected protein has conductivity <50µS and is substantially free from non-protein agents, which improves spot resolution and greatly reduces spot streaking. Perfect-FOCUS™ treated protein samples are compatible with mass spectrometry analysis and show identical peptide spectra. The Perfect-FOCUS™ kit is suitable for concentrating and cleaning protein samples for isoelectric focusing (IEF) and 2D-gel electrophoresis applications.

Protein samples containing interfering agents, including ionic detergents, metal ions, substrates, substrate analogs, inhibitors, and other charged molecules, routinely result in smeared or low resolution gels.