To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Description:PAGE-Perfect™
- Cat. no.:CA95057-536
- Supplier No.:786-123T
Specifications
About this item
Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis
PAGE-Perfect™ is a simple, two-step method for concentrating, cleaning and preparing protein solutions for running publication quality gels. Treat (1-100µL) protein solution with Universal Protein Precipitation Agent (UPPA™), which results in quantitative precipitation of the protein solution. Protein precipitation is not affected by the presence of detergents, chaotropes, or other common laboratory agents. The protein precipitate is collected by centrifugation and washed to remove any interfering agents such as detergents, salts, lipids, and other laboratory agents. Suspend the precipitate in the sample-loading buffer for loading on the gel for electrophoresis.
PAGE-Perfect™ allows for >99% protein recovery by removing electrophoresis interfering agents such as detergents, salts, chaotropes, reducing agents, and sugars. PAGE-Perfect™ concentrates and cleans dilute (>1ng/mL) protein samples to increase gel quality and reproducibility. Process 50x 1-100µL protein samples with PAGE-Perfect™.
The presence of contaminants, or interfering agents, such as salts, acids, bases and detergents, result in band distortion and poor protein resolution. As a result, SDS-PAGE gels are hard to analyze and lack reproducibility. PAGE-Perfect™ removes electrophoretic contaminants for improved protein resolution and publication quality gels. It is ideal for difficult or dilute samples.