About this item
This unique combination of proprietary buffer, stabilizers, and AccuFast™ Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, and specificity, along with a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR
- 2× concentrated reagents minimize pipetting steps and improve accuracy
- Ultrapure, hot start AccuStart enzyme technology and anti-foaming technology
- FastMix formulation supports both fast and standard thermal cycling conditions
AccuFast™ Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a unique mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (>48 hours at room temperature). Similar to our AccuStart™ Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast™ Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200bp is complete in less than 20 seconds at 60°C.
Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye. Platforms not requiring passive reference dye include Roche LightCycler® 480, Bio-Rad® iQ™, MyiQ™, iQ™5, Opticon®, and Chromo4™, Corbett Rotor-Gene®, Eppendorf® Mastercycler®, and Cepheid® SmartCycler®. Kits with Low ROX™ reference dye are for use with platforms including AB 7500 and Stratagene® Mx™. Kits with ROX™ reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne™.
Highly specific amplification is crucial to successful qPCR with SYBR® Green I dye technology because this dye binds to and detects any dsDNA generated during amplification.
To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Application:qPCR dye