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Specifications
- Concentration:120000 units/ml
- Description:Bst 2.0 DNA polymerase, glycerol-free
- Size:8000 units
- Cat. no.:CA77532-244
Specifications
About this item
Bst 2.0 WarmStart DNA polymerase (glycerol-free) is formulated without glycerol to support lyophilization, incorporation into microfluidic devices, and enable quick adoption into automation workflows.
- Glycerol-free format to support lyophilization and automation workflows
- Consistent amplification performance with the convenience of room temperature setup
- WarmStart technology eliminates off-target amplification and offers increased reaction efficiency
- Higher salt tolerance and thermostability compared to wild-type Bst DNA polymerase, large fragment
- Optimal reaction performance from 60 to 72 °C
Bst 2.0 WarmStart® DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA polymerase I, large fragment (Bst DNA polymerase, large fragment) with a reversibly-bound aptamer which inhibits polymerase activity at temperatures below 45 °C. At temperatures above 45 °C, the aptamer rapidly releases from the enzyme with no special activation step needed to activate the polymerase. Bst 2.0 WarmStart® DNA Polymerase contains 5' to 3' DNA polymerase activity and strong strand-displacement activity but lacks 5' to 3' exonuclease activity. It displays improved amplification speed, yield, salt tolerance, and thermostability compared to wild-type Bst DNA Polymerase, Large Fragment.