eQo 1-Step ToughMix®

No compromises. Unparalleled performance meets sustainability.

• Unrivaled sensitivity - detection down to two copies of RNA
• Sustainable and earth friendly: Lyophilized eQo bead format eliminates dry ice, CO₂ consumptions and reduces shipping costs
• Novel reverse transcriptase: Convenient, ambient reaction setup, overcomes rigid RNA secondary structure
• High stability: Up to one year at room temperature, up to one year rehydrated at −20 °C
• Tough Tested: Broad and significantly enhanced tolerance to PCR inhibitors

eQo 1-step ToughMix is a lyophilized reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes. It is supplied with a proprietary rehydration buffer that when combined with the lyophilized 'eQo beads' produces a stabilized 4X concentrated master mix.

The kit includes thermolabile UDG for amplicon carryover elimination, an enhanced warm-start reverse transcriptase (RT) and all other required components for 1-step RT-qPCR except RNA template and primer/probe(s). The reaction chemistry has been optimized for inhibitor tolerance and delivers exceptional performance in either single-plex or highly demanding multiplex 1 step RT-qPCR formats.

Cycler compatibility: BioRad CFX, Quantabio Q, Roche LightCycler 480