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Specifications
- Description:EnzyFluo™ Farnesyltransferase activity assay kit
- Size:400 tests
- Cat. no.:77441-680
Specifications
About this item
For quantitative determination of FTase enzyme activity in biological samples.
- Safe and convenient
- Non-radioactive assay
- 'Mix-incubate-measure' type assay
- No wash and reagent transfer steps are involved
- Sensitive and accurate
- Linear detection range 0.024 to 3.2 U/L FTase in a 384-well plate assay
- High-throughput
- Can be readily automated to assay thousands of samples per day
FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).