For quantitative determination of antioxidant capacity in food, beverages and biological samples.

• Sensitive and accurate
• Detects 11 to 300 μM Trolox equivalents in 96-well plate
• Stable reagent
• DPPH in this kit is included as a powder form that can be aliquoted to test as many or few wells as desired, or repeated testing over separate days, or weeks
• Simple and high-throughput
• This room temperature, homogenous, 'mix-incubate-measure' assay can be run in as little as 10 minutes
• Can be readily automated to assay thousands of samples per day

An antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants, such as the small molecules glutathione and vitamins or the macromolecules catalase and glutathione peroxidase, protect the cells from damage by reactive oxygen species. Antioxidants are widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline.

Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. BioAssay systems improved antioxidant capacity assay is based on the reduction of DPPH by antioxidant molecules in a sample, which results in a drop in absorbance at 517 nm. The antioxidant capacity can be quantified and expressed using a trolox standard.