Mouse Recombinant MSP1E3D1-His (from E. coli)

Membrane scaffold protein, mouse origin.

Extinction coefficient (in water) ε280: 29,400 M⁻¹cm⁻¹ (Human origin), 32,430 M⁻¹cm⁻¹ (Mouse origin), 36,440 M⁻¹cm⁻¹ (Rat origin)
Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
Affinity tag: N-Terminal his-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 (His-tagged) creates nanodiscs of 12 to 14 nm in diameter. This product contains a his-tag, keep that in mind when choosing the affinity tag for your membrane protein.

This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Mouse
Amino acid number:
277 (Human origin), 276 (Mouse origin), 267 (Rat origin);
Protein synonyms:
Membrane Scaffold Protein 1E3D1, His
Protein/peptide name:
MSP1E3D1-His
Purity:
> 90% (Determined by SDS-PAGE)
Molecular weight:
32600 Da (Human origin), 22534 Da (Mouse origin), 31572.76 Da (Rat origin)
Sequence:
GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ