Human Recombinant MSP1D1 dH5-His (from E. coli)

Membrane scaffold protein, human origin.

Extinction coefficient (in water) ε280: 19,940 M⁻¹cm⁻¹
Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 dh5 (his-tagged) creates nanodiscs of 7 to 8 nm in diameter. This product contains a his-tag, keep that in mind when choosing the affinity tag for your membrane protein.

This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
Storage of lyophilized copolymer: −20 °C for several years; Storage of reconstituted protein: 2...8 °C for several day
Amino acid number:
184
Protein synonyms:
Membrane Scaffold Protein 1D1, dH5-His
Protein/peptide name:
MSP1D1 dH5-His
Purity:
>90% (Determined by SDS-PAGE)
Molecular weight:
21468 Da
Sequence:
MGSSHHHHHH ENLYFQ GSTFSKLREQ LGPVTQEFWD NLEKETEGLR QEMSKDLEEV AKVQPYLDDF QKKWQEEMEL RQKVEPLGEE MRDRARAHVD ALRTHLAPYS DELRQRLAAR LEALKENGGA RLAEYHAKAT EHLSTLSEKA KPALEDLRQG LLPVLESFKV SFLSALEEYT KKLNTQ
Shipping temperature:
RT