Human Recombinant MSP1D1-His (from E. coli)

Membrane scaffold protein, human origin.

Extinction coefficient (in water) ε280: 25440 M⁻¹cm⁻¹ (Human origin), 22,460 M⁻¹cm⁻¹ (Mouse origin), 33460 M⁻¹cm⁻¹ (Rat origin), 41,940 M⁻¹cm⁻¹ (Alpaca origin)
Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
Affinity tag: N-Terminal his-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 (His-tagged) creates nanodiscs of 9 to 10 nm in diameter. This product contains a his-tag, keep that in mind when choosing the affinity tag for your membrane protein.

This product is available with different species as its background. This is important for e.g. for secondary antibodies of a western blot.

Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
Storage of lyophilized copolymer: −20 °C for several years; Storage of reconstituted protein: 2...8 °C for several day
Amino acid number:
217 (Human origin), 214 (Mouse origin), 210 (Rat origin), 232 (Alpaca origin)
Protein synonyms:
Membrane Scaffold Protein 1D1, His
Protein/peptide name:
MSP1D1-His
Purity:
>90% (Determined by SDS-PAGE)
Molecular weight:
25309 Da (Human origin), 24928 Da (Mouse origin), 24614 Da (Rat origin), 26743 Da (Alpaca origin)
Sequence:
MGSSHHHHHH ENLYFQ GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
Shipping temperature:
RT