About this item
Our PureCube Co-NTA XL agarose resins are agarose resin beads with a diameter of ∼400 µm. They are used for the purification of active His-tagged proteins from cells.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His tagged proteins
- Binding capacity: ∼30 mg/ml
- Bead ligand: Co-NTA
- Bead size: 400 μm
- Filling quantity: Delivered as a 50% suspension
Our year-long experience in manufacturing agarose resin lead to the high yield of 30 mg protein per ml resin, which is leading in the market compared to other Co-NTA suppliers. PureCube Co-NTA resins are suited for batch spin columns and FPLC. Their huge diameter provides great flow rates for even the most vicious cell media.
Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
To order chemicals, medical devices, or other restricted products, please provide identification that includes your business name and shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of identification are:
- • Issued document with your organization's Federal Tax ID Number
- • Government issued document with your organization's Resale Tax ID Number
- • Any other Government ID that includes the business name and address
Avantor will not lift restrictions for residential shipping addresses.
Supplier: VWR International
Supplier: BDH
Supplier: VWR International
Supplier: Corning
Supplier: VWR International
Supplier: VWR International