Our PureCube Ni-IDA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: >70 mg/ml
• Bead ligand: Ni-IDA
• Bead size: 40 μm
• Filling quantity: Delivered as a 50% suspension
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Our year-long experience manufacturing agarose resins led to a high yield of 70 mg protein per ml resin, leading in the market compared to other Ni-IDA suppliers. PureCube Ni-IDA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability. For higher flow rates in the case of extremely vicious cell media, we recommend our Ni-IDA XL agarose beads with 400 µm diameter. Furthermore, it is also possible to get these Ni-IDA beads pre-packed in a column/cartridge or as magnetic beads.

Required equipment:
- Lysis buffer
- Wash buffer
- Elution buffer
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment; Optional: Western - Blot reagents and equipment