À propos de cet article
PureCube maleimide MagBeads XL are your best option to couple biomolecules carrying thiol (SH) groups.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His tagged proteins
- Binding capacity: >80 mg/ml
- Bead ligand: Ni-NTA
- Bead size: 100 μm
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- Filling quantity: Delivered as a 50% suspension
Required equipment and materials:
- FPLC hardware like e.g. ÄKTA
- Binding buffer
- Elution buffer
The resulting Thioether bond between the maleimide MagBeads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube maleimide activated MagBeads are also available as agarose resin in case you prefer this method catalog, or as MagBeads with a smaller bead diameter if your downstream application requires more mechanical stable beads.
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- • Issued document with your organization's Federal Tax ID Number
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- • Any other Government ID that includes the business name and address
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