Our PureCube Cu-NTA agarose resins are small agarose resin beads with a diameter of 40 µm.

• Usage: Specific binding and purification of 6× His-tagged proteins, Binding of Copper-binding proteins
• Specificity: Affinity to His-tagged proteins, affinity to Copper-binding proteins
• Binding capacity: >80 mg/ml
• Bead Ligand: Cu-NTA
• Bead size: 40 μm
• Filling quantity: Delivered as a 50% suspension
• Metal ion capacity: >15 µeqv Cu²⁺/ml
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
• Required equipment:
• Lysis Buffer
• Wash Buffer
• Elution Buffer
• Ice bath
• Refrigerated centrifuge for 50 ml tube (min 10000×g)
• 50 ml centrifuge tube
• Micropipettor and Micropipetting tips
• Disposable gravity flow columns with capped bottom outlet, 2 ml
• pH meter
• End-over-end shaker
• SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

PureCube Cu-NTA agarose resins are used for the purification of active His-tagged proteins from cells and the enrichment of Copper-binding proteins. Our year-long experience in manufacturing agarose resin led to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Cu-NTA suppliers. PureCube Cu-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with great mechanical stability.