The protein purification with the PureCube Glutathione agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

• Usage: Specific binding and purification of GST-tagged proteins
• Specificity: Affinity to GST(Glutathione-S-Transferase)-tagged proteins
• Binding capacity: >10 mg/ml
• Bead Ligand: L-Glutathione (reduced)
• Bead size: 40 μm
• Matrix: 7.5% cross-linked agarose
• Filling quantity: Delivered as a 50% suspension
• pH Stability: 3 to 12
• Required equipment and materials:
• FPLC hardware like e.g. ÄKTA
• Binding Buffer
• Elution Buffer

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. The PureCube cartridge/column used here is compatible with almost all commonly used FPLC systems like ÄKTA. The PureCube Glutathione Compact cartridge is an FPLC column pre-packed with PureCube Glutathione Agarose. Alternatively, Glutathione MagBeads are also available if you prefer this affinity chromatography approach. Additional support products for GST-tag purification assays are: L-glutathione (reduced), essential compound of the elution buffer, GST-tag antibodies for western blot detection.