HIV-1 Trimeric GP140 protein-clade A, BG505 strain (His tag)

À propos de cet article

A recombinant trimeric form of the HIV-1 glycoprotein (GP protein) from BG505 strain (clade A, GenBank: ABA61516.1) was produced by human embryonic kidney HEK293 cells, followed by Lectin purification. This product contains ectodomain of GP protein (end at D664) with furin cleavage site replaced by a linker, a T332N mutation for glycan, a I559P mutation for stable trimer formation, and C-terminal 8 hexa-histidine tag.

This product has been evaluated by Size Exclusion Chromatography (SEC) to confirm the trimer formation, SDS-PAGE gel for purity and ELISAs for binding, see results. Due to the unique feature of BG505 at residue 241 glycan hole, a rabbit antibody 11B can target this protein (ref: Cell Rep. 2016;16(9):2327-38.), but not other HIV GP with 241N, such as YU2 or 16055 trimer.

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Spécifications

Protein/peptide type:
Recombinant
Source:
HEK293 cells
Storage conditions:
–80 °C
Endotoxin-free:
No
Carrier-free:
Yes
Protease-free:
No
Animal-free:
Yes
Protein/peptide name:
HIV-1 Trimeric GP140 protein-clade A, BG505 strain
Molecular weight:
~ 98 kDa
Concentration:
0.2 mg/ml
Formulation:
Frozen Liquid. In PBS pH 7.5
Nuclease-free:
No
Shipping temperature:
Dry Ice
Tested applications:
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe