Nucleofector® Basic Kit for Primary Mammalian Endothelial Cells
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Product Details & Documents
In addition to various cell type-specific kits for primary endothelial cells, we also provide a cell group-specific basic Nucleofector® kit.
- Optimization can be performed within 1 experiment
- Detailed protocol guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
- Transfection efficiencies up to 90%
- Viabilities up to 85%
This kit is suited to determine the optimal transfection conditions for any mammalian endothelial cell type that lacks a cell type specific optimized protocol for the Nucleofector® 2b device (or older generations I or II). In a first experiment a selection of different Nucleofector® Programs is tested in combination with the basic Nucleofector® solution for endothelial cells.
Applications: Kit suited for endothelial cells from different mammalian species and various organs
Already tested for human pulmonary artery endothelial cells.
Delivery information: Content: 2.25 ml Nucleofector® solution for primary mammalian endothelial cells, 0.5 ml Supplement 1, 30 µg pmaxGFP® Vector (0.5 μg/μl in 10 mM Tris pH 8.0), 25 aluminum cuvette (100 µl), 25 single use pipettes.
Spécifications
Spécifications
- Désignation:Basic Nucleofector® kit for primary mammalian endothelial cells
- No. of Reactions:25
Spécifications
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Catalog #: CA21905-026
Supplier: Kimberly-Clark