Human Bone Marrow MNCs, Cryopreserved

Bone marrow mononuclear cells (BMNC) are a mixed population of single nucleus cells including monocytes, lymphocytes, and hematopoietic stem and progenitor cells.

• Test negative for mycoplasma, bacteria, yeast, and fungi
• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. Isolating mononuclear cells eliminates erythrocytes and granulocytes, producing a more stable cell product.

Lonza BMNCs are available fresh* or frozen from a large and varied donor pool.

Lonza offers choices in cell type and tissue source so you can choose the right product for your research and budget needs. Depending upon your ultimate cell of choice, you can choose from unprocessed bone marrow, bone marrow MNCs, bone marrow CD34+ cells, bone marrow stromal cells (for use as feeder cells), or bone marrow mesenchymal stem cells.

Recommended Media and Reagents:
HPGM® Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application). Alternative media containing serum may also be acceptable.

Fresh cells are suspended in HBSS with 5 mM EDTA and 0.5% BSA and are available in a variety of cells per vial packaging sizes.

Certifications: Lonza guarantees the performance of Clonetics®/Poietics® cells only if appropriate Clonetics®/Poietics® media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Delivery information: Content: 1×cryopreserved ampule BMNCs containing ≥5 million cells. A Certificate of Analysis is provided for each cell lot purchased.