VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

• For the detection and quantification of RNA targets using the SYBR®/FAM channel
• Ready-to-use kit containing all necessary reaction components, except for primers and RNA template
• Suitable for a variety of RNA sample types
• Includes Hot Start Taq DNA Polymerase and VWR® Reverse Transcriptase for improved reaction specificity
• Incorporates dUTP for carryover contamination prevention
• VWR® One-Step Reaction Mix is supplied at a 2X concentration

The VWR® RT Enzyme Mix, supplied at a 20X concentration, contains the specially designed, temperature-controlled VWR® Reverse Transcriptase and Murine RNase Inhibitor to assist in preventing RNA degradation. This temperature-controlled RT enzyme prevents unwanted non-specific activity and displays superior thermostability in comparison to other reverse transcriptases.