Rat 5-Hydroxytryptamine ELISA Kit

Rat 5-Hydroxytryptamine ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of rat 5-Hydroxytryptamine in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-To-Use ELISA Kit
• Detection Range: 1.563 - 100 ng/ml
• Sensitivity: 0.938 ng/ml
• Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Rat 5-Hydroxytryptamine ELISA kit (A73778) employs the competitive enzyme immunoassay technique for the quantitative measurement of rat 5-Hydroxytryptamine in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with 5-Hydroxytryptamine antigen. During the incubation, 5-Hydroxytryptamine present in the samples or standards competes with the fixed amount of immobilized 5-Hydroxytryptamine for binding sites on the Biotinylated Anti-5-Hydroxytryptamine Antibody. The more 5-Hydroxytryptamine present in a sample or standard, the less Biotinylated Anti-5-Hydroxytryptamine Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-5-Hydroxytryptamine Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of 5-Hydroxytryptamine present in each sample or standard. The concentration of 5-Hydroxytryptamine can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.