Recharge Supplement, without glucose and L-glutamine, is a chemically defined, animal origin-free (AOF) protein-free supplement designed to increase protein yields, while maintaining acceptable viability and cell growth.

• A chemically defined (CD) supplement to increase protein yields, while maintaining acceptable viability and cell growth
• Developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors
• Use in CHO mammalian cell culture systems, as a media supplement or a culture system feed
• Has been beneficial for non-CHO mammalian systems and microbial systems
• Can be used as a replacement for yeast extract

The supplement has been developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. In order to increase versatility of the formulation, glucose and L-glutamine are not included in the final formulations. Thereforce, these two components should be supplements by the user and monitored as appropriate.