VWR® PCR Buffers

An optimal PCR buffer system is critical for the performance of successful PCR.

• Choose between 3 PCR buffers

Key Buffer: Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) and minimizes the need for optimization of the Mg²⁺ concentration or the annealing temperature in most primer-template systems.

Extra Buffer: Extra Buffer is the traditional potassium (K+) buffer. Extra Buffer promotes high specificity. Careful optimization of primer annealing temperatures and Mg²⁺ concentrations may be required.

Combination Buffer: Combination Buffer is a proprietary mixture of K+ and NH⁴⁺. It combines high specificity with good product yield and high tolerance for optimization of primer annealing temperatures and Mg²⁺ concentrations due to its balanced ammonium-potassium formulation.

Long term storage at -20 °C. Product expiry at -20 °C is stated on the label. Optional: Store at +4 °C for up to 6 months.

VWR® Taq DNA Polymerase and VWR® TEMPase Hot Start DNA Polymerase kits generally include either one or two different PCR buffers which are suited for different PCR needs. All PCR buffers contain Tris and 15 mM MgCl₂ (1.5 mM MgCl₂ final concentration).

 : For Research Use Only. Not for use in diagnostic procedures.