Q5 Blood direct 2X master mix can amplify a wide variety of targets direct from dried blood spots to up to 30% whole human blood, skipping DNA purification. The master mix includes polymerase and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with Na EDTA, K2 EDTA, Na citrate and Na heparin as well samples stored on common preservative filter papers.

• Amplify direct from whole blood cells or dried blood spots without DNA extraction or purification
• Features Q5 hot start high-fidelity DNA polymerase for accurate and efficient PCR
• Optimized buffer for increased resistance to inhibitors and chemicals
• Consistent performance with a variety of filter types and anti-coagulants present
• Aptamer-based enzyme control enables room temperature reaction setup

The master mix features Q5 hot start high-fidelity DNA Polymerase, a thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain for robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.