The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.

• Includes NEBNext FS (Fragmentation System) enzymatic fragmentation reagents
• Save time: optimized PCR-free workflow, reduced hands-on time and automation compatibility
• Generate high quality libraries without an amplification step with inputs from 50 to 500 ng
• Improved library uniformity
• For use with the NEBNext Unique Dual Index UMI Adaptors DNA Set 1

The fast, user-friendly workflow includes enzymatic fragmentation for optimal input size, requires minimal hands-on time, and maintains excellent library yields and GC coverage without requiring PCR. Don’t be biased! The NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina® can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs®, you can trust that your samples are in the best hands – your own.