VSV-Pseudovirus_Zaire Ebola Luciferase, ReVacc Scientific

À propos de cet article

This pseudotyped virus uses recombinant vesicular stomatitis virus (rVSV) to carry GP protein of Zaire ebolavirus Makona strain (GenBank: KR781609.1).

Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See the titration result showing the undiluted pseudovirus generates signal over 5,000-fold higher than uninfected control (cell alone as background).

As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Applications:
•Anti- Ebola neutralizing antibody screening at high throughput
•Anti- Ebola drug screening at high throughput
•Ebola pseudovirus transduction of target cells for viral entry and functional studies

See suggested protocol to use this product.

Packaging: Shipping with dry ice. Require –80 °C storage. Multiple freeze/thaw cycles will reduce its sensitivity. Recommend only 1 cycle. Aliquot after the first thaw.

Caution: Handle it in biosafety cabinet in BSL-2. Contacted tips and tubes should be decontaminated by 10% disinfecting bleach. For research use only.

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